Aberrant Expression of Wnt/beta-catenin Signaling Pathway and in - Vitro Cytotoxic Activity of Tradescantia Spathacea Medicinal Plant Used to Treat Human Breast Adenocarcinoma (mcf-7 Cell Lines)

Cancer plays crucial role in human life and critical disease to cure. Signaling pathways involved in stem cells development process. Especially, Wnt/βcatenin signaling pathway plays important role in stem cells self-renewal, differentiation and proliferation. Despite, over expression and mutation of Wnt/βcatenin signaling pathway induced variety of cancer development. Anticancer activities of several natural and synthetic agents were used to treat many cancer types. Many synthetic agents were used to cure various cancer but they have their toxicity. So, plant derived chemotherapeutic agents are going to investigate against cancer without side effect. In this paper we evaluate anticancer properties of Tradescantia spathacea medicinal plant and aberrant expression of β-catenin protein in human breast adenocarcinoma cell line (MCF-7). The plant leaves were collected, shade dried and extracted with hydroalcohol using soxhlet. Cytotoxic activities were assayed with MTT colorimetric procedure against MCF-7 cell lines. From the result, plant leaves extracts showed nearly 50 % MCF-7 cell line inhibition at 229.7μg/ml tested dose. INTRODUCTION: Natural products from plants have been valuable sources for anticancer drug discovery 1 . A screening program was initiated by Leven et al., (1979) that identified many antibacterial antifungal, antiviral, antiparasitic, and other pharmacologically active substance activities in higher plants 2 . Herbal or ‘botanical’, medicines, recorded in developing countries with ancient civilizations, such as Egypt and China, provide an abundant Pharmacopoeia of products that have been prescribed for many diseases over many centuries. The natural products underlying traditional medicines have received increased scientific attention recently 3, 4 . QUICK RESPONSE CODE DOI: 10.13040/IJPSR.0975-8232.5(12).5230-34 Article can be accessed online on: www.ijpsr.com DOI link: http://dx.doi.org/10.13040/IJPSR.0975-8232.5(12).5230-34 Since there are national and indigenous rights over plant derived resources, basic scientific investigations based on medicinal plants and indigenous medical systems have increased in developing countries 3, 5 . Ancient herbal medicines may have some advantages over single purified chemicals 4 . Often the different components in an herb have synergistic activities or buffer toxic effects. Mixtures of herbs are even more complex and so might have more therapeutic or preventive activity than single products alone. In fact, several studies have demonstrated that extracts from several herbal medicines or mixtures had an anticancer potential in-vitro or in-vivo 5-8 . Phenolic and flavonoid contents provide antioxidant activities that may underlie the anticancer potential 9 . The cytotoxic screening models are the preliminary methods for selection of active plant extracts against cancer. A huge